Presented in 2012, the RNAscope is one of the more recent RNA in situ hybridization techniques that allows visualization of multiple target genes. RNAscope uses a probe design strategy that amplifies the signal and suppresses the background, which results in increased sensitivity and specificity, see [1].
The protocol automatically detects and quantifies probes associated with a cell nucleus in dermis and epidermis, respectively. No manual outlining of dermis and epidermis is needed, since these regions are automatically identified.