In magnetic resonance imaging (MRI) a contrast agent is used to enhance soft tissue of interest. In a study, see [1], a method was developed to detect a nanoparticle based contrast agent, specifically designed to target tumor tissue. Polyethylene glycol (PEG) coats the nanoparticles and can be targeted by antibodies through immunohistochemistry (IHC) staining, using diaminobenzidine (DAB).
This APP automatically detects the PEG positive areas in the tissue as well as the ratio compared to the total tissue area, which should give an indication of the tumor growth.