Pulmonary fibrosis is the formation or development of excess fibrous connective tissue (fibrosis) in the lungs. Pulmonary fibrosis is a gradual exchange of normal lung parenchyma with fibrotic tissue, causing irreversible decrease in oxygen diffusion capacity. In addition, decreased compliance makes pulmonary fibrosis a restrictive lung disease. It is the main cause of restrictive lung disease that is intrinsic to the lung parenchyma. With Masson’s trichrome, the collagen in a rat lung section is stained blue and therefore very easy to distinguish from other tissue types. A magnification of 5X and a random 50% sampling is sufficient for an accurate and robust estimation of the collagen to tissue ratio.
Rat, Pulmonary Fibrosis
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