Developed for Akoya Biosciences, Inc.
The widely used biomarker panel for lung cancer (CD8, PD-1, PD-L1, CK, CD68, FoxP3 and CD68) was developed to understand immune infiltration in the face of a PD-L1 blockade. It can be utilized to assess (a) the change in the immune interaction across the tumor-stroma interface and (b) the composition on either side of the normal-tumor invasive edge. Phenotypes are identified using the Multiplex Phenotyping module. This APP is designed to work with amplified fluorescence signals that have been unmixed with multispectral imaging, in which each stain is spectrally isolated from both spectrally overlapping fluorophores and background autofluorescence.