Mammalian sodium/proton exchangers (Na+/H+) and the Na+, HCO3–cotransporter (NBCN1) in MCF-7 human breast cancer cells, function in intracellular pH (pHi) and cell volume homeostasis by catalyzing an electroneutral exchange of extracellular sodium and intracellular hydrogen. NBCn1 exerts net acid extrusion, could contribute to malignant cancer phenotypes in the same manner as NHE1.
This APP can be used for quantification of NBCN1 membrane immunoreactivity. The parameters used in the APP allow measurement of the expression of sodium/proton exchangers at the plasma membrane in tissue sections. As the function of sodium/proton exchangers relates to their localization, quantitative assessment of localized expression will presumably correlate with function. Comparison of matched sets of samples (e.g. tumors versus normal) allows correlation of expression as a function of (disease) status.
NBCN1, Breast Cancer
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