This protocol can be used for evaluation of nervous tissue. Nerves consist of sensory, motor, and autonomic nerve axons. Particularly those of larger diameter, are surrounded by myelin. This APP can quantify axon cross-sectional area, myelin thickness, and the G-ratio (axon major axis length / myelin major axis length), which is relevant for the study of neurodegenerative diseases and a variety of neuropathies.
The protocol automatically detects cross sections of nerves. Each nerve section is evaluated for large, small, and degenerated nerve fibers. The small and large nerve fibers are partitioned into axon and myelin and important parameters, as major axis length, are exported per axon.