The widely used biomarkers for breast cancer: ER, PR, Ki-67, and HER2 are combined with four CD biomarkers (CD3, CD4, CD8, and CD20) in a virtual fluorescence 8-plex multiplex setting. Two serial sections are stained with the breast cancer biomarkers and CD biomarkers, respectively, and aligned using our patented Tissuealign™ module. While maintaining a 4-plex staining method, all 8 biomarkers are displayed simultaneously in the aligned image. Due to co-expressers, this assay yields a total of 12 different phenotypes that can all be detected with the “10141 – Virtual 8-plex Multiplexing, Breast Cancer, TME” APP.
Virtual 8-plex Multiplexing, Breast Cancer, TME
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